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THE APPLICATION OF THE PERCOLATION THEORY FOR THE DESCRIPTION OF THE PLASMA-MEMBRANE INTEGRAL PROTEIN MOBILITY AND THE HORMONE-STIMULATED ADENYLATE-CYCLASE ACTIVITY .2. THE INFLUENCE OF POLYLYSINE UPON HORMONE-STIMULATED ADENYLATE-CYCLASE ACTIVITYстатья
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Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
- Авторы: ZAKHAROVA OM, ROSENKRANZ AA, SOBOLEV AS
- Журнал: Biologicheskie Membrany
- Том: 11
- Номер: 4
- Год издания: 1994
- Издательство: Biologicheskie Membrany
- Местоположение издательства: Russian Federation
- Первая страница: 412
- Последняя страница: 419
- Аннотация: The influence of polylysine upon hormone-stimulated adenylate cyclase activity in rat reticulocyte plasma membrane was studied. It was shown that the hormone-stimulated adenylate cyclase activity decreased down to the catalytic unit activity (activity in the presence of 20 mM Mn2+) with the increasing polylysine concentration. It was found that the adenylate cyclase activity also decreased with the increasing polylysine molecular weight at the constant residue concentration. Treatment of membranes with polylysine led to a decrease in the fraction of beta-adrenergic receptor with high affinity as it was shown by ligand binding characteristics of beta-adrenergic agonist isoproterenol. By using the technique of fluorescence recovery after photobleaching we found that the pretreatment of plasma membrane with polylysine resulted in a decrease in the lateral diffusion coefficient and the mobile fraction of integral membrane proteins. It was shown that the results of studying of the polylysine influence upon the hormone-stimulated adenylate cyclase activity and the integral protein mobylity could be interpreted in terms of the percolation theory.
- Добавил в систему: Розенкранц Андрей Александрович