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Convergent Synthesis of Oligosaccharides Structurally Related to Galactan I and Galactan II of Klebsiella Pneumoniae and their Use in Screening of Antibody Specificityстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 сентября 2019 г.
- Авторы: Argunov Dmitry A., Trostianetskaia Anastasiia S., Krylov Vadim B., Kurbatova Ekaterina A., Nifantiev Nikolay E.
- Журнал: European Journal of Organic Chemistry
- Том: 26
- Год издания: 2019
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 4226
- Последняя страница: 4232
- DOI: 10.1002/ejoc.201900389
- Аннотация: Klebsiella pneumoniae Is a Gram‐negative pathogenic bacterium that emerges a challenge for modern medicine due to intensively rising multi‐drug resistance. Specific LPS O‐chains represent promising targets for development of vaccines for immunoprophylaxis or immunotherapy of K. pneumoniae‐caused diseases. Herein, the synthesis of di‐, tetra‐, and hexasaccharides which represent structural fragments of both galactan I and galactan II of K. pneumoniae LPS O‐chains is described. The galactofuranoside building block used for assembling the galactan I chains was prepared by pyranoside‐into‐furanoside (PIF) rearrangement of the corresponding galactopyranoside precursor bearing an acid‐labile temporary p‐methoxybenzyl protecting group at O(3). The synthesized set of K. pneumoniae antigenic oligosaccharides in the form of their biotinylated derivatives was used to create a glycoarray which was applied for the screening of antibodies to galactan I and galactan II in anti‐K. pneumoniae sera. The obtained results demonstrate the applicability of such a glycoarray‐based assay for typing antibodies to Klebsiella strains belonging to most frequent O1 serogroup.
- Добавил в систему: Trostianetskaia Anastasiia Sergeevna