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The application of the percolation theory for the description of the plasma-membrane integral protein mobility and the hormone-stimulated adenylate-cyclase activity. 1. the influence of change in the fluid lipid fraction…статья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
- Авторы: Zakharova OM, Akhlynina TV, Rosenkranz AA, Sobolev AS
- Журнал: Biologicheskie Membrany
- Том: 11
- Номер: 4
- Год издания: 1994
- Издательство: Biologicheskie Membrany
- Местоположение издательства: Russian Federation
- Первая страница: 402
- Последняя страница: 411
- Аннотация: The influence of ''fluid'' lipid fraction changes upon the mobility of membrane integral proteins and the hormone-stimulated adenylate cyclase activity was studied in rat reticulocyte plasma membrane. By using the technique of fluorescence recovery after photobleaching it was found that decreasing of the ''fluid'' lipid fraction induced by the treatment of plasma membrane with phospholipase A2 resulted in a decrease in the lateral diffusion coefficient and in the mobile fraction of integral membrane proteins from 0,74.10(-10) cm2/s to 0,27.10(-10) cm2/c and 62,8 to 25,8% respectively. By using radioligand techniques the intrinsic activities of agonists: isoproterenol, adrenalin, noradrenalin,- and their ability for stimulating the hormone - beta-adrenergic receptor - G(s)-protein complex formation decreased as the ''fluid'' lipid fraction was reduced. It was shown that the hormone-stimulated adenylate cyclase activity and the plasma membrane integral protein mobility could be described in terms of the precolation theory.
- Добавил в систему: Розенкранц Андрей Александрович