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On the regulative role of the glutamate receptor in mitochondriaстатья Исследовательская статья
- Авторы: Selin Alexey A., Lobysheva Natalia V., Nesterov Semen V., Skorobogatova Yulia A., Byvshev Ivan M., Pavlik Lyubov L., Mikheeva Irina B., Moshkov Dmitry A., Yaguzhinsky Lev S., Nartsissov Yaroslav R.
- Журнал: Biological Chemistry
- Том: 397
- Номер: 5
- Год издания: 2016
- Издательство: Walter de Gruyter GmbH & Co. KG
- Местоположение издательства: Germany
- DOI: 10.1515/hsz-2015-0289
- Аннотация: The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was shown to be suppressed by γ-aminobutyric acid (GABA). The GABA receptor presence in the heart mitochondria was shown by golding with the use of antibodies to α- and β-subunits of the receptor. The activity of glutamate receptor was assessed according to the rate of synthesis of hydrogen peroxide. The glutamate receptor in mitochondria could be activated only under conditions of hypoxic stress, which in model experiments was imitated by blocking Complex I by rotenone or fatty acids. The glutamate signal in mitochondria was shown to be calcium- and potential-dependent and the activation of the glutamate cascade was shown to be accompanied by production of hydrogen peroxide. It was discovered that H2O2 synthesis involves two complexes of the mitochondrial electron transfer system - succinate dehydrogenase (SDH) and fatty acid dehydrogenase (ETF:QO). Thus, functions of the glutamate signaling system are associated with the system of respiration-glycolysis switching (the Pasteur-Crabtree) under conditions of hypoxia.
- Добавил в систему: Нестеров Семён Валерьевич