Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplastsстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:In this work, we investigated the interaction of a number of novel pH-sensitive spin probes (imidazoline- and imidazolidine-based nitroxides) with isolated chloroplasts. The light-induced changes in the electron paramagnetic resonance signals of these radicals are determined by several factors: Delta pH-driven accumulation of nitroxides inside the thylakoids, consumption (or evolution) of oxygen by chloroplasts, and irreversible loss of spin probe paramagnetism. A comparative study of these radicals characterized by different pK(a) allowed us to conclude that these spin probes can be used as pH indicators for monitoring the intrathylakoid pH- and light-induced changes in oxygen concentration in chloroplast suspension.