Photosynthetic electron transport in the cyanobacterium Synechocystis sp PCC 6803: High-field W-band and X-band EPR study of electron flow through photosystem Iстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:In this work, by using the respective advantages of W- and X-band electron paramagnetic resonance (EPR) spectroscopy techniques to investigate electron transport processes, we have studied the light-induced redox transients of the primary electron donor P-700, and the secondary acceptor A, in photosystem (PS) I complexes of intact cyanobacterial cells Synechocystis sp. PCC 6803. We found that the kinetic behavior of the cation radical P-700(center dot+). generated by illumination with continuous light, and the EPR intensity of the radical pair P-700(center dot+), A(1)(center dot-), generated upon laser pulse illumination strongly depend on the illumination prehistory (either the sample was frozen in the dark or during illumination). Both these processes were sensitive to the presence of electron transport inhibitors which block electron flow between the two photosystems. In line with our X-band EPR data on the kinetics of light-induced redox transients of P-700, our high-field W-band EPR study of the radical-pair state P(700)(center dot+)A(1)(center dot-) shows that photosynthetic electron flow through the PS I reaction center is controlled both on the donor and on the acceptor side of PS.