Extraction and Determination of Biologically Active Components of St. John’s Wort and Its Pharmaceutical Preparationsстатья
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Дата последнего поиска статьи во внешних источниках: 1 августа 2016 г.
Аннотация:A procedure is developed for the determination of biologically active substances (BAS) of common
St. John’s wort (Hypericum perforatum L.) by HPLC using two columns, Luna C18, 100 Å (for the determination
of phenolcarboxylic acids and flavonoids), and Onyx Monolithic C18 (for the determination of phloroglucinols
and naphthodianthrones), in the gradient elution mode with diode array detection. The detection
limits for analytes are 0.05–0.20 μg/mL. To optimize the conditions, we studied the extraction of biologically
active substances from St. John’s wort by a water–ethanol solution at different temperatures and pressures
and under the effect of microwave radiation and ultrasound. The maximum amounts of substances were
extracted in a dynamic mode under heat and pressure. The procedure was applied to the St. John’s wort samples
of different brands and some pharmaceutical products based on it. The components of extracts were
identified by retention times, UV spectra, and mass spectra. It was shown that the content of biologically
active substances in pharmaceutical samples of St. John’s wort depends on the herb habitat. It was shown that
hyperforin decomposed in pharmaceutical formulations based on St. John’s wort during storage.