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Apoenzymes of Alkaline Phosphatases of Different Origin for Determining of Trace Zinc(II)статья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Zhavoronkova A.M., Muginova S.V., Shekhovtsova T.N.
- Журнал: Journal of Analytical Chemistry
- Том: 58
- Номер: 6
- Год издания: 2003
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 594
- Последняя страница: 600
- DOI: 10.1023/A:1024180606247
- Аннотация: The applicability of the apoenzymes of three alkaline phosphatases of different origin (bacterial from E. coli and animal from chicken intestine or the small intestine of a Greenland seal) to the determination of zinc(II) was studied. The alkaline apophosphatase from seal intestine was found to be promising for this purpose. The preparation conditions of the apoenzyme and the conditions of its reactivation with zinc(II) were optimized. Based on the reactivating effect of zinc(II) on the apoenzyme, which was prepared with the use of EDTA, an enzymatic method was developed for determining zinc(II) (0.01-0.1 μg/mL) with cmin = 8 ng/mL. The proposed procedure was applied to the determination of zinc(II) in blood serum.
- Добавил в систему: Шеховцова Татьяна Николаевна