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Functional analysis of movement proteins encoded by Hibiscus green spot virusстатья Тезисы
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 января 2020 г.
- Авторы: Solovyev A., Lazareva E., Lezzhov A., Morozov S., Heinlein M.
- Журнал: FEBS open bio
- Том: 9
- Номер: Suppl. 1
- Год издания: 2019
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 310
- DOI: 10.1002/2211-5463.12675
- Аннотация: Viral cell-to-cell transport in plants requires specialized virus-encoded movement proteins (MPs), which provide delivery of viral genomes to plasmodesmata and their further translocation to neighboring cells. Genomes of many plant viruses carry 'triple gene block' (TGB), a conserved gene module coding for three MPs termed TGB1, TGB2 and TGB3. Recently, we have demonstrated that the genome of Hibiscus green spot virus (HGSV, the genus Higrevirus) contains a novel transport module, a ‘binary movement block’ (BMB), consisting of two MP genes designated as BMB1 and BMB2. The HGSV BMB1 and BMB2 proteins are necessary and sufficient for cell-to-cell transport of a reporter virus construct. Evolutionary, BMB is distantly related to TGB. Similarly to TGB1, BMB1 has a helicase domain; however it is closer in sequence to viral replicative helicases than to TGB1 proteins. BMB2, like both TGB2 and TGB3, is a highly hydrophobic protein, which has a marginal sequence similarity to TGB2 and is functionally similar to TGB3, combining therefore features of the two TGB proteins. Transient co-expression of BMB1 and BMB2 fused to fluorescent proteins has revealed that the BMB2 protein can direct BMB1 to peripheral membrane bodies, which are derivative of the endoplasmic reticulum (ER) membranes located in close vicinity of plasmodesmata, to the plasmodesmata interior, and to neighboring cells. BMB2-directed transport to plasmodesmata-associated sites depends on functional integrity of the ER/actin network and can occur via lateral translocation along the ER membranes. Collectively, these data demonstrate that plant virus cell-to-cell movement is closely associated with ER membranes and is accompanied by modifications of ER structures.
- Добавил в систему: Соловьев Андрей Геннадьевич