Precursors of particular miRNAs are present in the phloem of Cucurbita maximaстатьяТезисы
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Дата последнего поиска статьи во внешних источниках: 23 января 2020 г.
Аннотация:Plant development and responses to environmental cues largely depend on mobile signals including microRNAs (miRNAs) required for post-transcriptional silencing of specific genes. Short-range cell-to-cell transport of miRNA in developing tissues and organs is involved in transferring positional information essential for determining cell fate. Among other RNA species, miRNAs are found in the phloem sap. Long-distance transport of miRNA via the phloem takes a part in regulation of physiological responses to changing environmental conditions. As
shown for regulation of inorganic phosphorus and sulfate homeostasis, mature miRNAs rather than miRNAs precursors are transported in the phloem as signaling molecules. Bioinformatics analysis of transcriptomic data for Cucurbita maxima long phloem RNAs was carried out to elucidate whether miRNA precursors could also be present in the phloem. 120 annotated Cucumis melo miRNA precursor sequences were used as queries for BLAST searches in C. maxima phloem transcriptome assembly. Obtained sequences were filtered by estimated sequencing coverage. Results contained several pre-miRNA sequences such as premiR159, 166, 319, 390, 393 and others. In addition, we demonstrated that the phloem transcriptome contained a subset of C. maxima pri-miRNAs that differed from a subset of pri-miRNAs in leaf mesophyll transcriptome identified using the same procedure. Differential accumulation of pri-miRNA was confirmed by PCR analysis of C. maxima phloem and leaf RNA samples. Therefore, this data indicate that a number of C. maxima pri-miRNAs
are selectively recruited to the phloem translocation pathway.