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[Heterologous expression of functionally active human cytochrome P-450. I. Synthesis of cytochrome P-450IID6 by transformed Saccharomyces cerevisiae cells]статья
Статья опубликована в журнале из списка RSCI Web of Science
Статья опубликована в журнале из перечня ВАКСтатья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Krynetski? E.I.u, Kovaleva I.E., El’darov M.A., Luzikov V.N.
- Журнал: Молекулярная биология
- Том: 27
- Номер: 5
- Год издания: 1993
- Первая страница: 1094
- Последняя страница: 1099
- Аннотация: Functionally active human cytochrome P-450IID6 was expressed in Saccharomyces cerevisiae cells transformed with yeast expression vectors containing a fragment of the 2 mu-plasmid and cytochrome P-450 cDNA. Transcription was regulated by yeast acid phosphatase PHO5 promoter or chimeric GAL10-CYC1 promoter. Induced yeast cells synthesized cytochrome P-450 that was revealed by the optical absorption of the reduced CO form. Intact yeast cells were capable to metabolize the drug sparteine forming biotransformation products usually found in human. Expression of the human cytochromes P-450 seems to be a promising route to investigate new drugs and to screen genotoxic compounds.
- Добавил в систему: Эльдаров Михаил Анатольевич