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A Novel Method of Covalent Lysozyme Immobilization for the Development of Materials for Medical Applicationsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 сентября 2019 г.
- Авторы: Levashov P.A., Matolygina D.A., Ovchinnikova E.D., Adamova I.Yu, Dmitrieva O.A., Pokrovsky N.S., Eremeev N.L.
- Журнал: Russian Journal of Bioorganic Chemistry
- Том: 45
- Номер: 2
- Год издания: 2019
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 101
- Последняя страница: 106
- DOI: 10.1134/s1068162019020055
- Аннотация: Different methods of covalent lysozyme immobilization have been compared to choose an optimal approach to the development of a material suitable for medical applications in extracorporeal therapy. A novel method for lysozyme immobilization on a polymeric agarose matrix has been proposed, which provides the effective action of lysozyme on bacterial cells and eliminates the leakage of the enzyme from the material. The resultant immobilized lysozyme exhibits bacteriolytic activity toward Gram-positive bacteria Micrococcus luteus and Gram-negative bacteria Escherichia coli. During the immobilization of the enzyme, a broadening of the pH optimum of its activity occurs. The compatibility of immobilized lysozyme with human whole blood has been shown.
- Добавил в систему: Левашов Павел Андреевич