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DEVELOPMENT OF A POLARIZATION FLUOROIMMUNOASSAY FOR SULFAMETHAZINE USING AN AUTOMATED ANALYZERстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: EREMIN SA, LANDON J., SMITH DS, JACKMAN R.
- Журнал: The Analyst
- Том: 119
- Номер: 12
- Год издания: 1994
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 2723
- Последняя страница: 2726
- DOI: 10.1039/an9941902723
- Аннотация: A rapid, non-isotopic, polarization fluoroimmunoassay for the antibiotic sulfamethazine has been developed. The influence of the structure of fluorescein-labelled tracers on the sensitivity of the assay was investigated. Reagents were adapted for use with the Abbott TDx Analyzer and existing software was adapted for automated analyses. Total time for the assay of 1 and 20 samples was 14 and 22 min, respectively. All analytical criteria for the assay were satisfied. The detection limit for sulfamethazine in 20 mu l of aqueous sample was 10 ng ml(-1) (200 pg), which compares well with the sensitivity of enzyme linked immunosorbent assay methods.
- Добавил в систему: Еремин Сергей Александрович