USE OF THE METHOD OF FLUORESCENCE POLARIZATION TO DETERMINE THE DEGREE OF ORDER IN THE ACTION OF AMYLASESстатья
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Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
Местоположение издательства:Road Town, United Kingdom
Первая страница:873
Последняя страница:877
Аннотация:The mechanism of the action of amylases on amylose labeled with fluorescein isothiocyanate was studied by the fluorescence polarization technique. The method permits the detection of differences in the degree of randomization of the hydrolysis catalyzed by amylases from various sources. Calibration curves reflecting the dependence of the fluorescence polarization on the enzyme activity were obtained.