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Rapid all-in-one three-step immunoassay for non-instrumental detection of ochratoxin A in high-coloured herbs and spicesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Goryacheva Irina Yu, De Saeger Sarah, Nesterenko Irina S., Eremin Sergei A., Van Peteghem Carlos
- Журнал: Talanta
- Том: 72
- Номер: 3
- Год издания: 2007
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 1230
- Последняя страница: 1234
- DOI: 10.1016/j.talanta.2006.12.049
- Аннотация: A feasible three-step method for ochratoxin A (OTA) rapid detection was developed and applied for OTA screening in high-coloured matrices such as liquorice, ginger, nutmeg, black pepper, white pepper and Capsicum spp. spices at a control level of 10 mu g kg(-1). The method was based on the clean-up tandem immunoassay column and involved three steps: extract application, washing step and application of chromogenic substrate. A significant simplification of the assay was reached by using an additional frit with conjugate inside the clean-up tandem immunoassay column. The time for analysis was less than 10 min, including 5 min for colour development. Results were visually evaluated as colour development for negative result or no colour development for positive result. The method was coupled with a simple methanol-based extraction. A total of 27 samples were screened for OTA with the proposed method. It was shown that two samples of red pepper and one sample of liquorice, pili-pili, chilli and cayenne were contaminated with OTA above the control level at 10 mu g kg(-1), but none of tested ginger, nutmeg, black pepper and white pepper. (C) 2007 Published by Elsevier B.V.
- Добавил в систему: Еремин Сергей Александрович