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Cap- and IRES-independent scanning mechanism of translation initiation as an alternative to the concept of cellular IRESsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Shatsky I.N., Dmitriev S.E., Terenin I.M., Andreev D.E.
- Журнал: Molecules and Cells
- Том: 30
- Номер: 4
- Год издания: 2010
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 285
- Последняя страница: 293
- DOI: 10.1007/s10059-010-0149-1
- Аннотация: During the last decade the concept of cellular IRES-elements has become predominant to explain the continued expression of specific proteins in eukaryotic cells under conditions when the cap-dependent translation initiation is inhibited. However, many cellular IRESs regarded as cornerstones of the concept, have been compromised by several recent works using a number of modern techniques. This review analyzes the sources of artifacts associated with identification of IRESs and describes a set of control experiments, which should be performed before concluding that a 5' UTR of eukaryotic mRNA does contain an IRES. Hallmarks of true IRES-elements as exemplified by well-documented IRESs of viral origin are presented. Analysis of existing reports allows us to conclude that there is a constant confusion of the cap-independent with the IRES-directed translation initiation. In fact, these two modes of translation initiation are not synonymous. We discuss here not numerous reports pointing to the existence of a cap- and IRES-independent scanning mechanism of translation initiation based on utilization of special RNA structures called cap-independent translational enhancers (CITE). We describe this mechanism and suggest it as an alternative to the concept of cellular IRESs.
- Добавил в систему: Дмитриев Сергей Евгеньевич