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Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed cropsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 октября 2019 г.
- Авторы: Chernova Alina, Mazin Pavel, Goryunova Svetlana, Goryunov Denis, Demurin Yakov, Gorlova Lyudmila, Vanyushkina Anna, Mair Waltraud, Anikanov Nikolai, Yushina Ekaterina, Pavlova Anna, Martynova Elena, Garkusha Sergei, Mukhina Zhanna, Savenko Elena, Khaitovich Philipp
- Журнал: PeerJ
- Том: 7
- Год издания: 2019
- Издательство: PeerJ
- Местоположение издательства: San Francisco
- Первая страница: e6547
- DOI: 10.7717/peerj.6547
- Аннотация: Oilseed crops are one of the most important sources of vegetable oils for food andindustry. Nutritional and technical properties of vegetable oil are primarily determinedby its fatty acid (FA) composition. The content and composition of FAs in plants arecommonly determined using gas chromatography-mass spectrometry (GS-MS) or gaschromatography-flame ionization detection (GC-FID) techniques. In the present work,we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)technique to FA profiling of sunflower and rapeseed seeds and compared this methodwith the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs inrapeseed, while UPLC-MS appeared to be more sensitive, detecting about 2.5 timeshigher numbers of FAs in both plants. In addition to even-chain FAs, UPLC-MS wasable to detect odd-chain FAs. The longest FA detected using GC-FID was an FA with24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with thetails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MShas great potential to be used for the assessment of FA profiles of oil crops.
- Добавил в систему: Горюнов Денис Валерьевич