Two- and three-photon absorption cross-section characterization for high-brightness, cell-specific multiphoton fluorescence brain imaging

Авторы: Lanin Aleksandr A., Chebotarev Artem S., Pochechuev Matvei S., Kelmanson Ilya V., Kotova Daria A., Bilan Dmitry S., Ermakova Yulia G., Fedotov Andrei B., Ivanov Anatoly A., Belousov Vsevolod V., Zheltikov Aleksei M.
Журнал: Journal of Biophotonics
Том: 12
Год издания: 2019
Издательство: Wiley - VCH Verlag GmbH & CO. KGaA
Местоположение издательства: Germany
DOI: 10.1002/jbio.201900243
Аннотация: We demonstrate an accurate quantitative characterization of absolute two- and three-photon absorption (2PA and 3PA) action cross sections of a genetically encodable fluorescent marker Sypher3s. Both 2PA and 3PA action cross sections of this marker are found to be remarkably high, enabling high-brightness, cell-specific two- and three-photon fluorescence brain imaging. Brain imaging experiments on sliced samples of rat’s cortical areas are presented to demonstrate these imaging modalities. The 2PA action cross section of Sypher3s is shown to be highly sensitive to the level of pH, enabling pH measurements via a ratiometric readout of the two-photon fluorescence with two laser excitation wavelengths, thus paving the way toward fast optical pH sensing in deep-tissue experiments. This article is protected by copyright. All rights reserved.
Добавил в систему: Ланин Александр Александрович

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