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The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 1 августа 2016 г.
- Авторы: Myasnikov A.G., Afonina Z.A., Menetret J.F., Shirokov V.A., Spirin A.S., Klaholz B.P.
- Журнал: Nature communications
- Том: 5
- Год издания: 2014
- Издательство: Nature Pub. Group
- Местоположение издательства: United Kingdom
- Первая страница: 5294
- DOI: 10.1038/ncomms6294
- Аннотация: During protein synthesis, several ribosomes bind to a single messenger RNA (mRNA) forming large macromolecular assemblies called polyribosomes. Here we report the detailed molecular structure of a 100 MDa eukaryotic poly-ribosome complex derived from cryo electron tomography, sub-tomogram averaging and pseudo-atomic modelling by crystal structure fitting. The structure allowed the visualization of the three functional parts of the polysome assembly, the central core region that forms a rather compact left-handed supra-molecular helix, and the more open regions that harbour the initiation and termination sites at either ends. The helical region forms a continuous mRNA channel where the mRNA strand bridges neighbouring exit and entry sites of the ribosomes and prevents mRNA looping between ribosomes. This structure provides unprecedented insights into protein- and RNA-mediated inter-ribosome contacts that involve conserved sites through 40S subunits and long protruding RNA expansion segments, suggesting a role in stabilizing the overall polyribosomal assembly.
- Добавил в систему: Спирин Александр Сергеевич