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ATP-synthase of Rhodobacter capsulatus: coupling of proton flow through F-0 to reactions in F-1 under the ATP synthesis and slip conditionsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Feniouk B.A., Cherepanov D.A., Junge W., Mulkidjanian A.Y.
- Журнал: FEBS Letters
- Том: 445
- Номер: 2-3
- Год издания: 1999
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 409
- Последняя страница: 414
- DOI: 10.1016/S0014-5793(99)00160-X
- Аннотация: A stepwise increasing membrane potential was generated in chromatophores of the phototrophic bacterium Rhodobacter capsulatus by illumination with short flashes of light, Proton transfer through ATP-synthase (measured by electrochromic carotenoid bandshift and by pH-indicators) and ATP release (measured by luminescence of luciferin-luciferase) mere monitored. The ratio between the amount of protons translocated by F0F1 and the ATP yield decreased,vith the flash number from an apparent value of 13 after the first flash to about 5 when averaged over three flashes, In the absence of ADP, protons slipped through F0F1, The proton transfer through F0F1 after the first flash contained two kinetic components, of about 6 ms and 20 ms both under the ATP synthesis conditions and under slip, The slower component of proton transfer was substantially suppressed in the absence of ADP, We attribute our observations to the mechanism of energy storage in the ATP-synthase needed to couple the transfer of four protons with the synthesis of one molecule of ATP, Most probably, the transfer of initial protons of each tetrad creates a strain in the enzyme that slows the translocation of the following protons, (C) 1999 Federation of European Biochemical Societies.
- Добавил в систему: Мулкиджанян Армен Яковлевич