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Brain-specific sequence Hfb1 is a part of the large 3 '-untranslated region of the human complexin 2 gene: New in vitro and in silico dataстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 9 сентября 2021 г.
- Авторы: Dergunova L.V., Raevskaya N.M., Vladychenskaya I.P., Poltaraus A.B., Klimov E.A., Udina I.G., Sulimova G.E., Limborska S.A.
- Журнал: Molecular Biology
- Том: 35
- Номер: 5
- Год издания: 2001
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 660
- Последняя страница: 666
- DOI: 10.1023/A:1012361901392
- Аннотация: A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 cosmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3'-untranslated region of the mRNA for synaptic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBLY15167) isolated previously from a cDNA library of the frontal cerebral cortex, the primary structure was established for genomic clone Ghfb sized more than 4 kb. A GenBank search revealed complete identity of the 5' end of Ghfb and the 3'-untranslated region (878-933) of the human complexin 2 mRNA. Large transcripts with the 5' end corresponding to the complexin 2 mRNA and the 3' end to Ghfb were detected in total mRNA of the human brain by means of RT-PCR. The size of the 3'-untranslated region of the human complexin 2 mRNA was estimated at 4 kb.
- Добавил в систему: Владыченская Ирина Петровна