[Glycoproteins from Drosophila melanogaster cell culture contains O-bound carbohydrate chains of the Gal(beta1-3)GalNAc type]

Авторы: Kramerov A.A., Arbatskiĭ N.P., Rozovskiĭ Ia M., Pochechueva T.I., Mikhaleva E.A., Druzhinina T.I., Gvozdev V.A., Shibaev V.N.
Журнал: Biochemistry (Moscow)
Том: 60
Номер: 5
Год издания: 1994
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 805
Последняя страница: 812
Аннотация: The glycoprotein from cultured cells of D. melanogaster, also detected in various insect tissues as a component of the extracellular matrix, was characterized as a mucin-type glycoprotein not yet described in invertebrates. This glycoprotein with an apparent molecular mass of approximately 90 kDa contains about 40% of carbohydrates, largely represented mainly by GalNAc and Gal; its polypeptide moiety is enriched with Thr, Ser, Pro and Gly. An analysis of oligosaccharides liberated by treatment of the glycoprotein with alkaline NaBH4 or O-glycanase (endo-alpha-N-acetylgalactosaminidase) revealed Gal(beta 1-3)GalNAc as the major type of the sugar chains. About half of the Thr + Ser residues in the glycoprotein were estimated as O-glycosidically linked with the disaccharide units.
Добавил в систему: Гвоздев Владимир Алексеевич

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