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High-resolution label-free 3D mapping of extracellular pH of single living cellsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 января 2020 г.
- Авторы: Yanjun Zhang, Yasufumi Takahashi, Sung Pil Hong, Fengjie Liu, Joanna Bednarska, Goff Philip S., Pavel Novak, Andrew Shevchuk, Sahana Gopal, Iros Barozzi, Luca Magnani, Hideki Sakai, Yoshimoto Suguru, Takuto Fujii, Peter Gorelkin, Ерофеев Александр Сергеевич, Alexander Majouga, Weiss Dominik J., Christopher Edwards, Ivanov Aleksandar P., David Klenerman, Sviderskaya Elena V., Edel Yuri Korchev &.Joshua B
- Журнал: Nature communications
- Том: 19
- Год издания: 2019
- Издательство: Nature Pub. Group
- Местоположение издательства: United Kingdom
- Первая страница: 152
- DOI: 10.1038/s41467-019-13535-1
- Аннотация: Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.
- Добавил в систему: Мажуга Александр Георгиевич