Biochemical changes in the visual system in mice models of Parkinson's disease

Kim, A.; Pavlova, E.; Bogdanov, V.; Safandeev, V.; Ugrumov, M.

Авторы: Kim A., Pavlova E., Bogdanov V., Safandeev V., Ugrumov M.
Сборник: FEBS Open Bio
Серия: 9 (Suppl. 1)
Том: 1
Год издания: 2019
Место издания: John Wiley & Sons Inc United States Poland
Первая страница: 233
DOI: 10.1002/2211-5463.12675
Аннотация: Parkinson's disease (PD), characterized by loss of nigrostriatal dopaminergic neurons, is diagnosed by the appearance of motor disorders many years after the onset of neurodegeneration, which explains low efficiency of treatment. The only hope to improve the treatment is the development of preclinical diagnosis, based on searching biomarkers as changes in premotor functions. Since impaired visual function is among PD manifestations, this study aimed to search for biochemical changes in the eye of mice when modeling preclinical (presymptomatic) and clinical (symptomatic) stages of PD using neurotoxin MPTP. The mRNA level and protein content of tyrosine hydroxylase (TH) were assessed in the retina using realtime PCR and Western blot. In addition, the concentration of catecholamines was measured in the retina and iridociliary complex by HPLC. Presymptomatic mice show the increased levels of dopamine and metabolites in retina. Apparently, this is due to a compensatory increase in TH activity, since the TH protein content remains at the control level. Symptomatic mice show an abrupt decrease in the TH protein content, whereas the retinal dopamine content remains at the control level. This observation is also a manifestation of a compensatory reaction. The above data suggest that compensatory changes observed in the retina are similar to those previously found in the substantia nigra in these animal models. In the iridociliary complex, a decrease in the norepinephrine concentration was found in symptomatic mice, which may be due to a degradation of noradrenergic neurons innervating the iris and ciliary body. Changes found in parkinsonian animals correlate well with clinical data that the reaction of the pupil is slowed and the incidence of glaucoma is increased in PD patients. Thus in presymptomatic mice, pathological processes extend to the eye, followed by changes in the tear fluid and intraocular pressure, which can serve as preclinical diagnostic markers.
Добавил в систему: Сафандеев Виталий Васильевич

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