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Three widely separated positions in the 16S RNA lie in or close to the ribosomal decoding region; a site-directed cross-linking study with mRNA analoguesстатья
Информация о цитировании статьи получена из
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Дата последнего поиска статьи во внешних источниках: 20 ноября 2019 г.
- Авторы: Dontsova O., Dokudovskaya S., Kopylov A., Bogdanov A., Rinke-Appel J., Jünke N., Brimacombe R.
- Журнал: EMBO Journal
- Том: 11
- Номер: 8
- Год издания: 1992
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 3105
- Последняя страница: 3116
- DOI: 10.1002/j.1460-2075.1992.tb05383.x
- Аннотация: Synthetic mRNA analogues were prepared by T7 transcription, each containing several thio-uridine residues at selected positions. After binding to the ribosome in the presence of cognate tRNA, the thio-U residues were activated by UV irradiation and the resulting sites of cross-linking to 16S RNA analysed. Three distinct cross-links were consistently observed: (i) from position '+6' of the mRNA (the 3'-base of the A-site codon) to base 1052 of 16S RNA; (ii) from position '+7' of the mRNA to base 1395; and (iii) from '+11' to base 532. Individual yields of the cross-links were strongly dependent on the particular mRNA sequence in each case. The '+11/532' and '+6/1052' cross-links were always entirely tRNA-dependent, whereas the '+7/1395' cross-link was observed at lower intensity in the absence of tRNA. In the presence of a second (A-site bound) tRNA the +6/1052 cross-link was markedly reduced. A cross-link to the 1050 region was again observed when a message carrying a thio-U at position '+9' was translocated on the ribosome so as to bring the thio-U to position +6. Taken together, the data are incompatible with some current models both for the three-dimensional arrangement of 16S RNA and for the orientation of the tRNA-mRNA complex in the ribosome.
- Добавил в систему: Донцова Ольга Анатольевна