A Search for Microscopic Fungi with Directed Hydroxylase Activity for the Synthesis of Steroid Drugs

Karpova, N.V.; Andryushina, V.A.; Stytsenko, T.S.; Druzhinina, A.V.; Feofanova, T.D.; Kurakov, A.V.

Авторы: Karpova N.V., Andryushina V.A., Stytsenko T.S., Druzhinina A.V., Feofanova T.D., Kurakov A.V.
Журнал: Applied Biochemistry and Microbiology
Том: 52
Номер: 3
Год издания: 2016
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 316
Последняя страница: 323
Оригинальные версии: Поиск перспективных гидроксилирующих культур для синтеза фармацевтических стероидов
DOI: 10.1134/S000368381603008X
Аннотация: The hydroxylase activities of new strains such as Curvularia lunata, C. geniculata, C. eragrostidis, C. prasadii, Ulocladium botrytis, Alternaria tenuis, and Fusarium oxysporum toward three steroid substrates, namely, androstenedione (AD), cortexolone (S), and dehydroepiandrosterone acetate (DAA), were characterized. The 9α-hydroxylase activity of C. lunata 1011 cells against S to form 9α-hydroxy-S was shown for the first time. It was found that C. geniculata 837 and F. oxysporum 11dn1 strains can hydroxylate substrates to form pharmacologically promising 7α-hydroxysteroids. C. geniculata 837 cells selectively hydroxylate AD, resulting in 7α-hydroxytestosterone, whereas F. oxysporum 11dn1 leads to the transformation of DAA to 7α-hydroxydehydroepiandrosterone. Keywords: screening of fungal strains, hydroxylation with fungal cultures, steroid substrates DOI: 10.1134/S000368381603008X The hydroxylase activities of new strains such as Curvularia lunata, C. geniculata, C. eragrostidis, C. prasadii, Ulocladium botrytis, Alternaria tenuis, and Fusarium oxysporum toward three steroid substrates, namely, androstenedione (AD), cortexolone (S), and dehydroepiandrosterone acetate (DAA), were characterized. The 9α-hydroxylase activity of C. lunata 1011 cells against S to form 9α-hydroxy-S was shown for the first time. It was found that C. geniculata 837 and F. oxysporum 11dn1 strains can hydroxylate substrates to form pharmacologically promising 7α-hydroxysteroids. C. geniculata 837 cells selectively hydroxylate AD, resulting in 7α-hydroxytestosterone, whereas F. oxysporum 11dn1 leads to the transformation of DAA to 7α-hydroxydehydroepiandrosterone.
Добавил в систему: Кураков Александр Васильевич

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

A Search for Microscopic Fungi with Directed Hydroxylase Activity for the Synthesis of Steroid Drugsстатья

Прикрепленные файлы