Местоположение издательства:Road Town, United Kingdom
Первая страница:1779
Последняя страница:1787
Аннотация:A method has been developed for measuring the rates of rotenone-sensitive
oxidation of NADH and oligomycin-sensitive hydrolysis of ATP in rat skeletal
muscle homogenates. The method is based on the use of alamethicin which increases
the permeability of the inner mitochondrial membrane for NADH and ATP. It has
been shown that prolonged cold adaptation of rats (4 weeks, 4 degrees) does not
change the activity of rotenone-sensitive NADH-oxidase in rat skeletal muscle
homogenates which is equal to 12.4 +/- 4.4 nmol NADH/min/mg protein, but
increases threefold that of F0F1-ATPase--from 31.8 +/- 7.4 up to 93.1 +/- 14.3
nmol P(i)/min/mg protein. It is suggested that prolonged cold adaptation induces
structural-and-functional changes in the H(+)-ATP-synthetase complex of skeletal
muscle mitochondria.