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Photosystem II Activity of Wild Type Synechocystis PCC 6803 and Its Mutants with Different Plastoquinone Pool Redox Statesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 сентября 2016 г.
- Авторы: Voloshina O.V., Bolychevtseva Y.V., Kuzminov F.I., Gorbunov M.Y., Elanskaya I.V., Fadeev and V.V
- Журнал: Biochemistry (Moscow)
- Том: 81
- Номер: 8
- Год издания: 2016
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 858
- Последняя страница: 870
- DOI: 10.1134/S000629791608006X
- Аннотация: To assess the role of redox state of photosystem II (PSII) acceptor side electron carriers in PSII photochemical activity, we studied submillisecond fluorescence kinetics of the wild type Synechocystis PCC 6803 and its mutants with natural variability in the redox state of the plastoquinone (PQ) pool. In cyanobacteria, dark adaptation tends to reduce PQ pool and induce a shift of the cyanobacterial photosynthetic apparatus to State 2, whereas illumination oxidizes PQ pool, lead ing to State 1 (Mullineaux, C. W., and Holzwarth, A. R. (1990) FEBS Lett., 260, 245248). We show here that darkadapt ed Ox– mutant with naturally reduced PQ is characterized by slower QA– reoxidation and O2 evolution rates, as well as lower quantum yield of PSII primary photochemical reactions (Fv/Fm) as compared to the wild type and SDH– mutant, in which the PQ pool remains oxidized in the dark. These results indicate a large portion of photochemically inactive PSII reaction centers in the Ox– mutant after dark adaptation. While light adaptation increases Fv/Fm in all tested strains, indicating PSII activation, by far the greatest increase in Fv/Fm and O2 evolution rates is observed in the Ox– mutant. Continuous illumination of Ox– mutant cells with lowintensity blue light, that accelerates QA– reoxidation, also increases Fv/Fm and PSII functional absorption crosssection (590 nm); this effect is almost absent in the wild type and SDH– mutant. We believe that these changes are caused by the reorganization of the photosynthetic apparatus during transition from State 2 to State 1. We propose that two processes affect the PSII activity during changes of light conditions: 1) reversible inactivation of PSII, which is associated with the reduction of electron carriers on the PSII acceptor side in the dark, and 2) PSII activation under low light related to the increase in functional absorption crosssection at 590 nm.
- Добавил в систему: Еланская Ирина Владимировна