|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
[Bacterial expression of water-soluble domain of Lynx1, endogenic neuromodulator of human nicotinic acetylcholine receptors]статья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Shulepko M.A., Liukmanova E.N., Kasheverov I.E., Dolgikh D.A., Tsetlin V.I., Kirpichnikov M.P.
- Журнал: Bioorganicheskaia khimiia
- Том: 37
- Номер: 5
- Год издания: 2011
- Первая страница: 609
- Последняя страница: 615
- Аннотация: Lynx1 expresses in the central nervous system and plays important role in a regulation of nicotinic acetylcholine receptors. Successful milligram-quantitive expression of ws-Lynx1 was achieved only in the case of its production in the form of cytoplasm inclusion bodies. Different conditions of ws-Lynx1 refolding for yield optimization were performed. The obtained recombinant protein was characterized by means of mass spectrometry and CD spectroscopy. The binding experiments on the nAChRs from Torpedo californica membranes revealed that ws-Lynxl is biologically active and blocks muscle nAChR with IC50-20-30 microM.
- Добавил в систему: Долгих Дмитрий Александрович