[Expression of neurotoxin II from Naja oxiana cobra venom in Escherichia coli in a hybrid form with thioredoxin]

Авторы: Liukmanova E.N., Shul’ga A.A., Arsen’eva D.A., Pluzhnikov K.A., Dolgikh D.A., Arsen’ev A.S., Kirpichnikov M.P.
Журнал: Bioorganicheskaia khimiia
Том: 30
Номер: 1
Год издания: 2004
Первая страница: 30
Последняя страница: 40
Аннотация: Neurotoxin II from the venom of cobra Naja oxiana is a short type alpha-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.
Добавил в систему: Долгих Дмитрий Александрович

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