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Purification and Partial Characterization of a 14 kDa Protein Enhancing Mitogen-Stimulated Proliferation of Human Peripheral Blood Mononuclear Cellsстатья
- Авторы: Paromov V.M., Bughlava S., Malakhova N.V., Kiselevsky M.V., Dolgikh D.A., Kirpichnikov M.P.
- Журнал: Russian journal of immunology : RJI : official journal of Russian Society of Immunology
- Том: 4
- Номер: 2
- Год издания: 1999
- Издательство: The Journal
- Местоположение издательства: Moscow, Russia (Federation)
- Первая страница: 123
- Последняя страница: 130
- Аннотация: Cytotoxicity of plasma free platelets or peripheral blood mononuclear cells (PBMCs) upon stimulation with Ca-ionophore A23187 at various (10(-9) - 10(-6) M) concentrations has been studied on human erythroid leukaemia cell line (K562). A 14 kDa protein, which did not display any cytotoxic activity to K562 cells, but stimulate PBMC proliferation has been isolated from the supernatant of plasma free platelets stimulated by 10(-7) M Ca-ionophore. The protein displayed a very slight mitogenic effect on PBMCs, but it enhanced proliferative response of PBMC stimulated by concanavalin A at suboptimal concentrations. The p14 N-terminal sequence ((1)VLERTXA(7)-) is identical to the region 99-103 residues of the human MHC class II antigen DQ-beta chain. Also, we identified this 14 kDa protein in the supernatant of PBMCs stimulated by 10(-7) M Ca-ionophore. Its N-terminal sequence (VLERTXA-) is identical to the one of p14 from the stimulated plasma-free platelet supernatant.
- Добавил в систему: Долгих Дмитрий Александрович