|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
YB-3 substitutes YB-1 in global mRNA bindingстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Lyabin D.N., Eliseeva I.A., Smolin E.A., Doronin A.N., Budkina K.S., Kulakovskiy I.V., Ovchinnikov L.P.
- Журнал: RNA Biology
- Год издания: 2020
- Издательство: Landes Bioscience
- Местоположение издательства: United States
- DOI: 10.1080/15476286.2019.1710050
- Аннотация: Y-box binding proteins are DNA- and RNA-binding proteins with an evolutionarily ancient and conserved cold shock domain. The Y-box binding protein 1 (YB-1) is the most studied due to its abundance in somatic cells. YB-1 is involved in a variety of cellular processes, including proliferation, differentiation and stress response. Here, using Ribo-Seq and RIP-Seq we confirm that YB-1 binds a wide range of mRNAs and globally acts as a translation inhibitor. Surprisingly, YBX1 knockout results in only minor alterations in the expression of other genes, mostly caused by changes in RNA abundance. But YB-3 mRNA is an exception: it is better translated in the absence of YB-1, thereby producing an increased amount of YB-3 and thus suggesting that its synthesis is under YB-1 negative control. We have shown that the set of mRNAs bound to YB-3 is strikingly similar to that of YB-1, and that the mRNA-binding by YB-3 is enhanced in the absence of YB-1, resulting in a similar global reduction of translation of bound mRNAs in YB-1-null cells. Thus, YB-3 acts as a substitute for YB-1 in mRNA binding and, probably, in global translational control.
- Добавил в систему: Елисеева Ирина Александровна