|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Isolation of high-molecular-weight activation complexes of initiator caspases in DNA damageстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 сентября 2020 г.
- Авторы: Zamaraev A.V., Egorshina A.Y., Lavrik I.N., Zhivotovsky B.D., Kopeina G.S.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 169
- Номер: 1
- Год издания: 2019
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 132
- Последняя страница: 140
- DOI: 10.1007/s10517-019-04663-1
- Аннотация: Initiation of apoptosis by chemotherapeutic drugs is one of the most effective approaches to the treatment of cancers. Caspases, the main enzymes of apoptosis, undergo activation to initiate cell death. Activation of initiator caspases requires their binding to special protein complexes. For elucidation of the mechanisms of apoptosis, these complexes should be isolated. However, their purification is challenging because they are formed in the cell in negligible amounts and rapidly degrade. We have developed an effective way to isolate caspase activation complexes formed in tumor cells in response to DNA damage. The method is based on combination of gel filtration with immunoprecipitation. The first stage is aimed at the separation of the high-molecular-weight caspase activation complexes and their monomeric forms, which allows increasing the efficiency of isolation of complexes at the second stage.
- Добавил в систему: Лазарев Алексей Евгеньевич