Stabilization of alkaline proteinase and cellulases via complex formation with chitosanстатья
Информация о цитировании статьи получена из
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Scopus
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:An effective approach to the stabilization of hydrolytic enzymes (alkaline proteinase and cellulases)via the complex formation with chitosan for their further use as detergent components has been developed.Interaction with chitosan results in a 35–50% increase in the level of catalytic activity of the enzymes after incubationfor 60 min under the conditions of detergent use (alkaline pH, increased temperature, the presence ofanionic surfactants) as compared to the system in the absence of chitosan both due to the enzyme stabilizationand the increase of the starting level of catalytic activity. A twofold decrease of the enzyme inactivation constantis observed under the aforementioned conditions in the case of alkaline proteinase. In the case of cellulase preparation,the method for the control of the concentration of the active enzyme in the system modeling syntheticdetergents has been suggested. The method is based on the enzymatic destruction of the stabilizing agent, chitosan,by enzymes of the cellulase complex. The destruction of chitosan removed the stabilizing effect, thusresulting in the inactivation of cellulases. The developed approaches allow for the widening of the field of thepossible application of enzymes as detergent components.