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Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Functionстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 апреля 2020 г.
- Авторы: Pak Valeriy V., Ezeriņa Daria, Lyublinskaya Olga G., Pedre Brandán, Tyurin-Kuzmin Pyotr A., Mishina Natalie M., Thauvin Marion, Young David, Wahni Khadija, Agustín Martínez Gache Santiago, Demidovich Alexandra D., Ermakova Yulia G., Maslova Yulia D., Shokhina Arina G., Eroglu Emrah, Bilan Dmitry S., Bogeski Ivan, Michel Thomas, Vriz Sophie, Messens Joris, Belousov Vsevolod V.
- Журнал: Cell Metabolism
- Том: 31
- Номер: 3
- Год издания: 2020
- Издательство: Cell Press
- Местоположение издательства: United States
- Первая страница: 642
- Последняя страница: 653.e6
- DOI: 10.1016/j.cmet.2020.02.003
- Аннотация: Hydrogen peroxide (H2O2) is a key redox intermediate generated within cells. Existing probes for H2O2 have not solved the problem of detection of the ultra-low concentrations of the oxidant: these reporters are not sensitive enough, or pH-dependent, or insufficiently bright, or not functional in mammalian cells, or have poor dynamic range. Here we present HyPer7, the first bright, pH-stable, ultrafast, and ultrasensitive ratiometric H2O2 probe. HyPer7 is fully functional in mammalian cells and in other higher eukaryotes. The probe consists of a circularly permuted GFP integrated into the ultrasensitive OxyR domain from Neisseria meningitidis. Using HyPer7, we were able to uncover the details of H2O2 diffusion from the mitochondrial matrix, to find a functional output of H2O2 gradients in polarized cells, and to prove the existence of H2O2 gradients in wounded tissue in vivo. Overall, HyPer7 is a probe of choice for real-time H2O2 imaging in various biological contexts.
- Добавил в систему: Тюрин-Кузьмин Петр Алексеевич