N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organisms

Bonchuk, A.; Kamalyan, S.; Mariasina, S.; Boyko, K.; Popov, V.; Maksimenko, O.; Georgiev, P.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 августа 2020 г.

Авторы: Bonchuk Artem, Kamalyan Sofia, Mariasina Sofia, Boyko Konstantin, Popov Vladimir, Maksimenko Oksana, Georgiev Pavel
Журнал: Scientific reports
Том: 10
Номер: 1
Год издания: 2020
Издательство: Nature Publishing Group
Местоположение издательства: United Kingdom
Первая страница: 1
Последняя страница: 10
DOI: 10.1038/s41598-020-59459-5
Аннотация: CTCF is the main architectural protein found in most of the examined bilaterian organisms. the cluster of the C2H2 zinc-finger domains involved in recognition of long DNA-binding motif is only part of the protein that is evolutionarily conserved, while the N-terminal domain (NTD) has different sequences. Here, we performed biophysical characterization of CTCF NTDs from various species representing all major phylogenetic clades of higher metazoans. With the exception of Drosophilides, the N-terminal domains of CTCFs show an unstructured organization and absence of folded regions in vitro. In contrast, NTDs of Drosophila melanogaster and virilis ctcfs contain unstructured folded regions that form tetramers and dimers correspondingly in vitro. Unexpectedly, most NTDs are able to self-associate in the yeast two-hybrid and co-immunoprecipitation assays. These results suggest that NTDs of CTCFs might contribute to the organization of ctcf-mediated long-distance interactions and chromosomal architecture.
Добавил в систему: Марьясина Софья Семеновна

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

N-terminal domain of the architectural protein CTCF has similar structural organization and ability to self-association in bilaterian organismsстатья Исследовательская статья

Прикрепленные файлы