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Efficient synthesis of lasso peptide pseudomycoidin proceeds in the absence of both the leader and the leader peptidaseстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 марта 2020 г.
- Авторы: Zyubko Tatyana, Serebryakova Marina, Andreeva Julia, Metelev Mikhail, Lippens Guy, Dubiley Svetlana, Severinov Konstantin
- Журнал: Chemical science
- Том: 10
- Номер: 42
- Год издания: 2019
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 9699
- Последняя страница: 9707
- DOI: 10.1039/c9sc02370d
- Аннотация: Bacterial lasso peptides are made from linear ribosomally synthesized precursors by specific cleavage at the leader-core junction site of the precursor by a dedicated protease recognizing the leader, followed by cyclisation of the newly formed N-terminus of the core part with a side chain of the internal aspartic or glutamic residue catalyzed by a macrolactam synthetase. The resulting structure has a tail that is threaded and fixed inside the cycle formed. Here, we characterize a new lasso peptide, pseudomycoidin, encoded by DSM 12442. The most surprising and unique feature of pseudomycoidin is that it can be produced from the ribosomally synthesized core part by a macrolactam synthetase, in the absence of the leader protease. The minimalism of the pseudomycoidin synthesis system makes it a powerful model to generate pseudomycoidin-based lasso-peptide libraries and to study the poorly understood process of lasso formation. We detected two additional pseudomycoidin modifications: phosphorylation of a terminal residue that was previously observed in another lasso peptide, followed by glycosylation, which was not observed heretofore. We speculate that these bulky C-terminal modifications may help maintain the threaded lasso topology of the compound synthesized by the macrolactam synthetase.
- Добавил в систему: Серебрякова Марина Васильевна