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Chitin derivatives as systems for metal nanoparticles stabilizationтезисы доклада
- Авторы: Shirokova L.N., Alexandrova V.A., Vihoreva G.A., Revina A.A.
- Сборник: Abstracts Book of «9th International Conference of the European Chitin Society», Venice, Italy, May 23-26, 2009
- Тезисы
- Год издания: 2009
- Первая страница: PO3-12
- Аннотация: Natural biopolymer chitin and its derivatives are of considerable interest for creation of various biomedical materials due to the unique combination of properties such as low-toxicity, biodegradability, biocompatibility and others. At the same time such polyelectrolytes are possible to use for stabilization of the other bioactive compounds and construction of new macromolecular systems for application in biology and medicine [1]. The main goal of this study was investigation of the chitin derivatives stabilization ability relatively to the silver nanoparticles (SNP), obtained by biochemical method [2] and ferrum nanoparticles (FNP) generated by radiation-chemical way [3] in reverse micelles of anionic surfactant (AOT). As it seen from the data we obtained at investigation of the SNP distribution on the sizes (fig.1) the most part of particles (75%) were micromicelles (1–3 nm). We have investigated the number of polyelectrolytes of chitin-chitosan row for stabilization of metal nanoparticles. Among them the anionic polyelectrolytes carboxymethylchitin (CMCh) and sulfate of chitosan (SCh) were suitable to form compatible and stable macromolecular systems with SNP. Such polycationic systems as chitosan, trimethylchitosan gave unstable mixtures with metal nanoparticles because of possible AOT-polyelectrolyte complex formation. In our investigations CMCh ( MW 220 kD) with carboxymethylation degree 1.0 and SCh (MW 50–80 kD) with sulfonation degree 1.4 were applied. It was shown that mixtures of metal nanoparticles and polyelectrolytes solutions at polymer concentration 0.5–2% were stable during a month. These investigations were carried out using UV-VIS and photon-correlation spectroscopy methods. By comparison of absorption spectra and size of particles in the initial components (solution of SNP, FNP and polyelectrolytes) and their mixtures during the definite period we drew a conclusion about the derived macromolecular system stability. To evaluate the sizes of SNP we used photon-correlation spectroscopy method. Formation of more large particles in the derived mixtures and lack of small particles (relatively to the initial solutions) was possible caused by aggregation of macromolecules with immobilized SNP due to electrostatic interaction and formation of hydrogen bonds (fig. 2). We used CMCh modified with SNP for formation of films. The antimicrobial activity of the films against Salmonella typhimurium TMLR66 and Staphilococcus aureus Wood 46 was determined using the special procedure. On analyzing the experimental data obtained it was concluded that the films formed from CMCh modified with SNP exhibited significantly more antibacterial activity than the same films without SNP. REFERENCES 1. Chitin and chitosan: production, properties and usage. Edited by K.G. Skryabin, G.A. Vikhoreva, V.P. Varlamov. - Moscow.: Science, 2002. — 368 p. 2. E.M. Egorova, A.A. Revina. / Colloids and surfaces. A: Physicochem. and Eng. Aspects. 2000. Vol. 168. P. 87–96. 3. A.A. Revina, O.G. Larionov, A.A. Volkov, O.V. Suvorova // Sorption and chromatographic processes. In print.
- Добавил в систему: Широкова Людмила Николаевна