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Determination of 3J, 3J coupling constants in 13C-labeled nucleic acids using constant-time HMQCстатья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Hu W., Bouaziz S., Skripkin E., Kettani A.
- Журнал: Journal of Magnetic Resonance
- Том: 139
- Номер: 1
- Год издания: 1999
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 181
- Последняя страница: 185
- DOI: 10.1006/jmre.1999.1785
- Аннотация: A novel 1H-13C correlated two-dimensional experiment, CT-HMQC-J, for the measurement of three-bond proton-phosphorus coupling constants in 13C-labeled DNA is described. The experiment is based on the intensity difference of 1H-13C cross peaks in the presence and absence of the proton-phosphorus coupling interaction during the constant-time period in HMQC experiment. The 3J(H, P) coupling constants can be easily extracted from the intensity ratios of the two experiments. The method has been applied to a uniformly 13C, 15N-labeled d(GGAGGAT) 7-mer DNA sample. The proton-phosphorus coupling constants determined from CT-HMQC-J, together with the other three-bond coupling constants, are used to determine beta and epsilon torsion angles. The introduction of beta and epsilon restraints has improved the convergence as well as the quality of d(GGAGGAT) structure.
- Добавил в систему: Скрипкин Евгений Алексеевич