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Proteinase Inhibitors From Buckwheat (Fagopyrum esculentum Moench) Seedsстатья Глава в книге
Информация о цитировании статьи получена из
Scopus,
Web of Science
Дата последнего поиска статьи во внешних источниках: 27 мая 2020 г.
- Авторы: Dunaevsky Yakov E., Khadeeva Natalya V., Vassilevski Alexander A., Domash Valentina I., Belozersky Mikhail A.
- Сборник: NUTS AND SEEDS IN HEALTH AND DISEASE PREVENTION. Second Edition. Edited by VICTOR R. PREEDY RONALD ROSS WATSON
- Глава в коллективной монографии
- Год издания: 2020
- Издательство: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Местоположение издательства: 24-28 OVAL RD, LONDON,ENGLAND, NW1 7DX
- Первая страница: 521
- Последняя страница: 532
- DOI: 10.1016/B978-0-12-375688-6.10031-3
- Аннотация: Proteinase inhibitors in buckwheat seeds (BWI) consist of two main groups - endogenous involved in the regulation of storage protein metabolism and а group of exogenous proteinase inhibitors capable of suppressing the growth and development of pathogenic microorganisms. Exogenous protease inhibitors are presented by two subgroups, i.e. anionic and cationic inhib¬itors. As consisted with their amino acid sequences all anionic inhibitors, as well as two of the cationic inhibitors (BWI-3c and BWI-4c) were shown to belong to the potato proteinase inhibitor 1 family. The 3D structure of the proteinase inhibitor BWI-2c determined by NMR spectroscopy and revealing two antiparallel α-helices stapled by disulfide bonds showed that BWI-2c represents a new family of proteinase inhibitors termed α-hairpinins with an unusual α-helical hairpin fold. Possibility of obtaining transgenic tobacco and potato plants resistant to bacterial infections and insects by introduction of the gene of serine proteinase inhibitor BWI-1a (ISP) from buckwheat seeds has been demonstrated. Stable retention and expression of the heterologous protective ISP gene has been demonstrated on a collection of individual transgenic tobacco lines maintained in aseptic culture for more than 10 years.
- Добавил в систему: Белозерский Михаил Андреевич