Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazineстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 27 ноября 2016 г.
Аннотация:We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing haptenfunctionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenylsulfonamido) butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL–1, which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable and universal approach for detection of chemical contaminants.