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Comparative Analysis of Transgenic Tobacco Plants with Different Heterologic Plant Defensive Genesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 июня 2020 г.
- Авторы: Khadeeva N.V., Yakovleva E.Yu, Korostyleva T.V., Istomina E.A., Dunaevsky Ya E., Sydoruk K.V., Belozersky M.A., Odintsova T.I., Bogush V.G., Kudryavtsev A.M.
- Журнал: Russian Journal of Genetics
- Том: 56
- Номер: 3
- Год издания: 2020
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 307
- Последняя страница: 316
- DOI: 10.1134/S1022795420030084
- Аннотация: In order to study the possibility of creating new plant forms resistant to phytopathogens, a collection of transgenic plants of model tobacco culture with new different plant protective genes was obtained by the agrobacterial transformation method. First an addition of a collection with serine proteinase inhibitor BWI-1a (ISP) from buckwheat with fragments of a spidroin gene as putable enhancer by vector constructions different designs was done. Secondly, transgenic plants with an antimicrobial peptide from sinthetic wheat Triticum kiharae and with defensine fromStellaria media. Comparative study of physiological characteristics of transgenic plants in biotests in vivo (with isolated leaves) and in vitro (with well biotests) was carried out. Regardless of the design of the vector construction, the target genes were expressed to a different extent in the tissues of all transgenic plants and their seed and vegetative progenies and gave their tissues antibacterial activity, indicating the synthesis of the functional protein. The introduction to the tobacco tissues of the heterologic plant protective genes of different nature that plants use in different defense mechanisms led to a similar increase in antibacterial activity of the transgenic tobacco tissues.
- Добавил в систему: Белозерский Михаил Андреевич