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A camelid VHH-based fluorescence polarization immunoassay for the detection of tetrabromobisphenol A in waterстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 ноября 2016 г.
- Авторы: Wang Kai, Liu Zhiping, Pei Ji, Liu Jianfeng, Eremin Sergei A., Li Qing X., Li Ji, Ting Xu
- Журнал: Analytical Methods
- Том: 8
- Номер: 39
- Год издания: 2016
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 7265
- Последняя страница: 7271
- DOI: 10.1039/c6ay01603k
- Аннотация: Tetrabromobisphenol A (TBBPA) is a widely used flame retardant and is classified as an endocrine disruptor. A convenient and sensitive fluorescence polarization immunoassay (FPIA) for TBBPA was developed with a camelid variable domain of heavy chain antibody (VHH) being specific for TBBPA. 3-(2,6-Dibromo-4-(2-(3,5-dibromo-4-hydroxyphenly)propan-2-yl))propanoic acid (hapten T1) was conjugated to the ethylenediamine fluorescein thiocarbamyl (EDF) to form a tracer that binds with anti-TBBPA VHH. The limit of detection and half-maximum inhibition concentration of TBBPA by FPIA were 5 ng mL-1 and 90 ng mL-1, respectively. This FPIA was used to detect TBBPA in water samples without any pre-treatment. The recovery of TBBPA from spiked water samples ranged from 73% to 102%, with coefficients of variant ranging from 7% to 14%. The FPIA exhibited high potential for rapid and accurate determination of TBBPA in real water samples.
- Добавил в систему: Еремин Сергей Александрович
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