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Optimization of Solid-Phase Synthesis of the 1-40 Beta-Amyloid and Preparation of Antibodies Revealing It under Immunoblotting Conditionsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 июля 2020 г.
- Авторы: Volkova TD, Koroev DO, Kamynina AV, Filatova MP, Avetisyan AV, Volpina OM
- Журнал: Russian Journal of Bioorganic Chemistry
- Том: 46
- Номер: 2
- Год издания: 2020
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 217
- Последняя страница: 222
- DOI: 10.1134/S1068162020020181
- Аннотация: The solid-phase synthesis and purification of the 1-40 sequence of the human beta-amyloid were optimized, resulting in a preparation of a product with a high yield and homogeneity more than 95%. The synthetic peptide is capable of forming oligomers. This fact was confirmed by electrophoresis in the polyacrylamide gel with a subsequent immunoblotting and fluorescence spectrophotometry using the thioflavin T dye. An available method for a production of the highly specific anti-beta-amyloid antibodies with a high titer was developed. These antibodies recognized both monomeric and oligomeric forms of the 1-40 peptide of beta-amyloid under the immunoblotting conditions.
- Добавил в систему: Аветисян Армине Врежовна