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Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milkстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 сентября 2020 г.
- Авторы: Raysyan Anna, Galvidis Inna A., Schneider Rudolf J., Eremin Sergei A., Burkin Maksim A.
- Журнал: Journal of Pharmaceutical and Biomedical Analysis
- Том: 189
- Год издания: 2020
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Номер статьи: 113450
- DOI: 10.1016/j.jpba.2020.113450
- Аннотация: A lateral flow immunoassay (LFIA) using latex-labeled group-specific polyclonal antibodies was developed for the rapid simultaneous detection of six macrolide antibiotics in the breast milk. The results of LFIA test could be interpreted visually and instrumentally. The visual limits of detection (vLOD) were 1, 50, 1, 10, 10, and 1000 ng/mL for roxithromycin, azithromycin, clarithromycin, erythromycin, dirithromycin, and oleandomycin in buffer, respectively. The calculated limits of detection (cLOD) were respectively 0.12, 2.1, 0.15, 1.4, 2.4, and 3.3 ng/mL. To avoid a strong influence of matrix-mediated breast milk of a very diverse and variable composition, a sample pretreatment was proposed. The six macrolides could be visually detectable in breast milk after 20-min pretreatment at their content of 10- 1000 ng/mL. The recovery rate from the spiked samples carried out using strip scanner device ranged from 71 % to 110%, and accuracy of the results was from 3% to 14% (RSD). The described rapid on-site diagnostic assay format can be useful for monitoring the content of antibiotics in breast milk during macrolide treatment to ensure safe breastfeeding of infants.
- Добавил в систему: Еремин Сергей Александрович
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