Oxidation of phenothiazine and phenoxazine by Cunninghamella elegans

Авторы: Sutherland J.B., Freeman J.P., Heinze T.M., Moody J.D., Williams A.J., Parshikov I.A., Zhang D.
Журнал: Xenobiotica
Том: 31
Год издания: 2001
Первая страница: 799
Последняя страница: 809
DOI: 10.1080/00498250110069140
Аннотация: 1. To determine the ability of fungi to metabolize sulphur- and oxygen-containing azaarenes, Cunninghamella elegans ATCC 9245 was grown in 125-ml flasks containing fluid Sabouraud medium. The cultures and controls were incubated at 28°C with shaking and dosed with 16.7 mM phenothiazine or phenoxazine. After incubation for 72h, the mycelia and filtrates were extracted with ethyl acetate and the combined residues analysed by high-performance liquid chromatography. Residual phenothiazine and phenoxazine were 21 and 22%, respectively, of the total UV absorbance at 254 nm. 2. The metabolites were identified by mass spectrometry and proton nuclear magnetic resonance spectroscopy. The fungus oxidized phenothiazine to phenothiazine sulphoxide, 3-hydroxyphenothiazine sulphoxide, phenothiazin-3-one, and 3-hydroxyphenothiazine and oxidized phenoxazine to phenoxazin-3-one. 3. Three of the four compounds produced by C. elegans from phenothiazine were identical to those produced by mammals, supporting the use of the fungus as a microbial model for drug metabolism.
Добавил в систему: Паршиков Игорь Альбертович

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