|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
The fungus Pestalotiopsis guepini as a model for biotransformation of ciprofloxacin and norfloxacinстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Parshikov I.A., Heinze T.M., Moody J.D., Freeman J.P., Williams A.J., Sutherland J.B.
- Журнал: Applied Microbiology and Biotechnology
- Том: 56
- Номер: 3-4
- Год издания: 2001
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 474
- Последняя страница: 477
- DOI: 10.1007/s002530100672
- Аннотация: The metabolism of the fluoroquinolone drugs ciprofloxacin and norfloxacin by Pestalotiopsis guepini strain P-8 was investigated. Cultures were grown at 28 °C in sucrose/peptone broth for 18 days after dosing with ciprofloxacin (300 µM) or norfloxacin (313 µM). Four major metabolites were produced from each drug; and these were purified by high-performance liquid chromatography and identified by mass spectrometry and proton nuclear magnetic resonance spectroscopy. Ciprofloxacin metabolites included N-acetylciprofloxacin (52.0%), desethylene-N-acetylciprofloxacin (9.2%), N-formylciprofloxacin (4.2%), and 7-amino-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (2.3%). Norfloxacin metabolites included N-acetylnorfloxacin (55.4% ), desethylene-N-acetylnorfloxacin (8.8%), N-formylnorfloxacin (3.6%), and 7-amino-1-ethyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (2.1%). N-Formylciprofloxacin and the four transformation products from norfloxacin are all known to be mammalian metabolites.
- Добавил в систему: Паршиков Игорь Альбертович