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Chitosan-g-oligo(L,L-lactide) Copolymer Hydrogel Potential for Neural Stem Cell Differentiationстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 октября 2020 г.
- Авторы: Revkova Veronica A., Grebenik Ekaterina A., Kalsin Vladimir A., Demina Tatiana S., Bardakova Kseniia N., Shavkuta Boris S., Melnikov Pavel A., Samoilova Ekaterina M., Konoplyannikov Mikhail A., Efremov Yuri M., Zhang Chao, Akopova Tatiana A., Troitsky Alexandr V., Timashev Peter S., Baklaushev Vladimir P.
- Журнал: Tissue Engineering - Part A.
- Год издания: 2020
- Издательство: Mary Ann Liebert Inc.
- Местоположение издательства: United States
- Номер статьи: ten.tea.2019.0265
- DOI: 10.1089/ten.tea.2019.0265
- Аннотация: We evaluated the applicability of chitosan-g-oligo(L,L-lactide) copolymer (CLC) hydrogel for central nervous system tissue engineering. The biomechanical properties of the CLC hydrogel were characterized and its biocompatibility was assessed with neural progenitor cells obtained from two different sources: H9-derived neural stem cells (H9D-NSCs) and directly reprogrammed neural precursor cells (drNPCs). Our study found that the optically transparent CLC hydrogel possessed biomechanical characteristics suitable for culturing human neural stem/precursor cells and was noncytotoxic. When seeded on films prepared from CLC copolymer hydrogel, both H9D-NSC and drNPC adhered well, expanded and exhibited signs of spontaneous differentiation. While H9D-NSC mainly preserved multipotency as shown by a high proportion of Nestin+ and Sox2+ cells and a comparatively lower expression of the neuronal markers βIII-tubulin and MAP2, drNPCs, obtained by direct reprogramming, differentiated more extensively along the neuronal lineage. Our study indicates that the CLC hydrogel may be considered as a substrate for tissue-engineered constructs, applicable for therapy of neurodegenerative diseases.
- Добавил в систему: Тимашев Петр Сергеевич