Fluorescence Methods for the Analysis of Microtubule/Microfilament Involvement in the Regulation of Endothelial Barrier Function

Shakhov, A.; Alieva, I.

Авторы: Shakhov Anton, Alieva Irina
Сборник: Fluorescence Methods for Investigation of Living Cells and Microorganisms
Глава в коллективной монографии
Год издания: 2020
Место издания: IntechOpen London, EC3R 6AF, UK
Первая страница: 1
Последняя страница: 15
DOI: 10.5772/intechopen.93524
Аннотация: The endothelial monolayer is located on the inner surface of blood vessels and provides an important barrier function. The endothelium is used to control the transport of metabolites and nutrients through the vessel wall, both towards the circulating blood and in the direction of the underlying tissues. This function is provided by cytoskeletal structures that generate contractile and tensile forces, which are in an equilibrium state in the intact endothelium. Rearrangements of the cytoskeleton are the structural basis of hyperpermeability, since in this case there are changes in the shape of cells and the formation of intercellular spaces, which ultimately leads to endothelial dysfunction. Deep understanding of fundamental structural basis of endothelial barrier function maintaining is crucial problem because this phenomenon is common for a number of pathological states and diseases (inflammation, asthma, sepsis, acute lung injury, ischemia, diabetes) and can lead to severe, and even fatal, organ dysfunction, as well as a complication upon the treatment, for example, by a number of anti-cancer pharmacological drugs. Microtubules and the actin cytoskeleton function in cooperation in normal endothelium and under conditions of the barrier loss. In this review, we describe the application of modern fluorescence methods for investigation and analysis of the individual characteristics of cytoskeletal elements whose reorganization affects endothelial permeability, to emphasize the role of microtubules/microfilament crosstalk in EC barrier regulation.
Добавил в систему: Алиева Ирина Борисовна

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Fluorescence Methods for the Analysis of Microtubule/Microfilament Involvement in the Regulation of Endothelial Barrier Functionстатья

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