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Evaluation of the ordering of membranes in multilayer stacks built on an ATR-FTIR germanium crystal with atomic force microscopy: The case of the H+, K+-ATPase-containing gastric tubulovesicle membranesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Ivanov D., Dubreuil N., Raussens V., Ruysschaert J.M., Goormaghtigh E.
- Журнал: Biophysical Journal
- Том: 87
- Номер: 2
- Год издания: 2004
- Издательство: Biophysical Society
- Местоположение издательства: United States
- Первая страница: 1307
- Последняя страница: 1315
- DOI: 10.1529/biophysj.104.041863
- Аннотация: Polarized attenuated total reflection Fourier transform infrared spectra were recorded on multilayer stacks of native gastric tubulovesicle membranes. The spectral intensity and linear dichroism were measured for average thicknesses ranging between 0 and 100 bilayers. Atomic force microscopy was used to investigate the orientation of the membranes at the top of the stack. Height profiles were obtained along randomly drawn lines and slopes were computed over various distances. Orientation distribution functions were obtained from the slopes and decomposed into Legendre polynomials. It was found that the second Legendre polynomials coefficient characterizing the membrane orientation was always larger than 0.9. It could therefore be concluded that the membrane tilt does not significantly contribute to the infrared dichroism, even for the largest thicknesses tested.
- Добавил в систему: Иванов Дмитрий Анатольевич